ABSTRACT
In this study, we investigated the main constituent, the predominant class and biological activity of the essential oil extracted from the leaves of Pimenta dioica and the pattern of the major constituent against larvae in the third stage of Aedes aegypti. For this reason, we extracted the oil by hydrodistillation, identified its components by gas chromatography coupled with mass spectrometry (GC/MS) and calculated the lethal concentration (LC50) of the larvicidal activity using the Reed-Muench method. The results show that the oil consists mainly of eugenol, in which the phenylpropanoid class predominated and the lethal concentration, LC50, was 38.86 µg mL-1at a confidence level of 2.25 µg mL-1, while the eugenol standard presented LC5079.75 µg mL-1at a confidence level of 2.10 µg mL-1. Given the facts, we conclude that the oil is more active than the standard and that it has the potential to replace chemical larvicides.
En este estudio, investigamos el constituyente principal, la clase predominante y la actividad biológica del aceite esencial extraído de las hojas de Pimenta dioica y el patrón del constituyente principal contra las larvas en la tercera etapa de Aedes aegypti. Por este motivo, extrajimos el aceite por hidrodestilación, identificamos sus componentes mediante cromatografía de gases acoplada a espectrometría de masas (GC/MS) y calculamos la concentración letal (CL50) de la actividad larvicida mediante el método Reed-Muench. Los resultados muestran que el aceite está constituido principalmente por eugenol, en el que predominó la clase fenilpropanoide y la concentración letal, CL50, fue de 38,86 µg.mL-1 a un nivel de confianza de 2,25 µg.mL-1, mientras que el estándar de eugenol presentó CL50 79,75 µg.mL -1 a un nivel de confianza de 2,10 µg.mL-1. Dados los hechos, concluimos que el aceite es más activo que el estándar y que tiene el potencial de reemplazar los larvicidas químicos.
Subject(s)
Oils, Volatile/pharmacology , Oils, Volatile/chemistry , Aedes/drug effects , Pimenta/chemistry , Larvicides , Biological Assay , Biological Products , Eugenol/analysis , Plant Extracts/pharmacology , Plant Extracts/chemistry , Plant Leaves , Monoterpenes/analysis , Larva , Gas Chromatography-Mass SpectrometryABSTRACT
Um dos maiores desafios deste século continua sendo a produção de alimentos. A questão não é apenas produzir alimentos, mas assegurar a qualidade dos produtos para o consumidor final. Objetivou-se neste trabalho avaliar a contaminação de ovos comercializados na cidade de São Luís/MA por Salmonella spp. Foram analisadas 80 amostras de ovos oriundos de oito diferentes pontos comerciais. Nos resultados das análises microbiológicas foi evidenciado que 100% das amostras possuíam ausência de Salmonella spp., porém, 35% estavam contaminadas por bactérias pertencentes à família Enterobacteriaceae. Apesar de não ter sido constatada a presença de Salmonella spp.nas amostras analisadas, baseando-se nos resultados conclui-se que ocorreram falhas durante o processo de higienização dos ovos comercializados na cidade de São Luís/ MA, sendo necessárias melhorias na manipulação e acondicionamento dos ovos comercializados, visando minimizar potenciais riscos à saúde do consumidor.
One of the largest challenges of this century, it continues to be the production of foods for the population. The question is not just to produce food, but also to assure the products quality for the final consumer. In this context, the quality control of food becomes necessary in the sector nutritional. This work has for goal to evaluate microbiological aspects of the eggs consumed in the city of São Luís/ MA. They were analyzed 80 samples of eggs derived of eight markets different. The results of the analyses microbiological evidenced that 35% of the samples analyzed was contaminated. That result showed that the microbiological quality of the eggs commercialized in some markets city of São Luís/MA, is not in satisfactory terms for the consumption, being, therefore a risk in potential for the consumer health.